Cloning and characterization of the DIR1 promoter from Eucommia ulmoides Oliv and its response to hormonal and abiotic stress

The lignans of Eucommia ulmoides have been extensively studied and shown to a dual mechanism regulating blood pressure. Studies that DIR1 is key gene in the biosynthetic pathway Oliv. In this study, 2000 bp upstream promoter sequence was cloned, part (1495 bp) its 5′-end truncated segment were constructed into pCAMBIA1391Z expression plasmid ß-glucuronidase (GUS). Agrobacterium-mediated genetic transformation produced stable transgenic tobacco lines. results showed although both full-length promoters could initiate GUS expression, their levels influenced by degree deletion at 5′ end. histochemical staining core region located containing transcription initiation site (TIS) within 212 bp. addition, responded environmental hormonal stressors, such as jasmonic acid (MeJA), abscisic (ABA), d-mannitol (drought mimic), high concentrations NaCl. seedlings, MeJA, d-mannitol, ABA activate promoter, whereas NaCl inhibit it. E. Oliv NaCl, activated had an inhibitory effect. summary, our findings provide theoretical basis for use plant engineering, indicating potential. Our study also presents novel insights lignan biosynthesis sheds light on mechanisms response stress. We successfully cloned function gene.